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KMID : 0880220070450030268
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Journal of Microbiology
2007 Volume.45 No. 3 p.268 ~ p.271
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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage
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Ryoo Sung-Weon
Park Young-Kil
Park Sue-Nie
Shim Young-Soo
Liew Hyun-Jeong
Kang Seong-Man
Bai Gill-Han
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Abstract
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In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
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KEYWORD
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proteomic analysis, Mycobacterium tuberculosis K-strain, human monocytic cell line U-937, 2-D PAGE, MALDI-TOF-MS, Mb1363, MT2656
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